MicroCal iTC200 System Getting Started Booklet 28-9911-91 AB 5 Introduction Introduction MicroCal iTC 200 System Getting Started is designed to introduce you to the basic operations of MicroCal iTC200 system and software 1. This booklet provides guidance through the basic steps in a MicroCal iTC200 experiment using. MicroCal microcalorimeters are used in a wide range of applications from basic research to the discovery and development of small molecule drugs, biotherapeutics and vaccines. Our customers include pharmaceutical, biotech, academic and governmental research institutions worldwide.
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Malvern MicroCal PEAQ ITC Automated
MicroCal PEAQ ITC Automated offers the highest sensitivity with automation for high throughput, label free measurements of binding affinity and thermodynamics for the study of biomolecular interactions.
Malvern MicroCal PEAQ ITC Automated
MicroCal PEAQ ITC Automated offers the highest sensitivity with automation for high throughput, label free measurements of binding affinity and thermodynamics for the study of biomolecular interactions.
Manufacturer | Malvern Panalytical |
---|---|
Product Series | Malvern MicroCal ITC |
Measurement principle | Isothermal Titration Calorimetry |
Application | Biomolecular Interactions, Protein Interactions & Binding Affinity |
Sample type | Proteins, Biomolecules |
Sample Throughput | upto 42 per 24h (4x96 well plates) |
Noise | 0.15 ncal/s |
Temperature Range | 2℃ to 80℃ |
Response Time | 8s |
Product Overview
MicroCal PEAQ ITC Automated combines the high sensitivity of the MicroCal PEAQ ITC with walkaway automation to meet the productivity needs of busy research and drug discovery laboratories. Temperature controlled 4 x 96 well plates and user guided software together enable exceptional sensitivity and high quality data with robust operation. ITC is an essential tool in drug discovery and enables the study of biomolecular interactions as well as changes in conformation such as protein unfolding. It can also be used to measure enzyme kinetics.
Microcal Software Windows 7
MicroCal PEAQ ITC Automated measures heat released or absorbed during biochemical binding events to calculate binding affinity (K D ), stoichiometry (n), enthalpy (ΔH) and entropy (ΔS) in a single experiment. The system uses Isothermal Titration calorimetry (ITC), a technique that requires NO labelling and minimal assay development.
The PEAQ ITC micro calorimeter comprises two cells — a sample cell in which the titration experiment is performed, and a reference cell which contains only water. A series of small aliquots of ligand are injected into the sample or protein solution. If binding occurs between the ligand and protein, heat changes of a few millionths of a degree Celsius are detected and measured until the binding reaction has reached equilibrium. The quantity of heat measured is directly proportional to the amount of binding which helps the user understand the nature of the interaction.
Benefits
All the benefits of the MicroCal PEAQ ITC plus:
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Fully automated, high throughput
The MicroCal ITC Automated is a configurable, low volume, highly sensitive isothermal titration calorimeter. Silent hunter 5 crack 1.2 skidrow download. It offers the convenience of unattended operation with the capacity to run four 96 well plates simultaneously.
User-friendly software
Intuitive software ensures efficient experimental design with optimised automation scripts for robust operation with a variety of sample types and conditions. Design tools assist in selecting experimental run parameters to deliver fast, reliable results. New experiments can be edited ‘on the run’ to increase productivity.
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Applications
Microcal Origin Software
- Characterise biomolecular interactions to confirm binding, stoichiometry and thermodynamics
- Study the interaction between proteins, antibodies, nucleic acids, lipids, drugs and inhibitors
- Drug discovery for hit validation, lead optimisation and mechanisms of action Measure enzyme kinetics
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